Chemical structures of the core regions of Campylobacter jejuni serotypes O:1, O:4, O:23, and O:36 lipopolysaccharides

Eur J Biochem. 1993 May 1;213(3):1017-27. doi: 10.1111/j.1432-1033.1993.tb17849.x.


Complete structures, including the location of N-acetylneuraminic acid (Neu5Ac) residues, were assigned for the core regions of Campylobacter jejuni serotypes O:1, O:4, and O:23 and O:36 lipopolysaccharides (LPS). In continuation of earlier studies, structure determinations of liberated oligosaccharides and, where necessary, of intact LPS, were by 1H-NMR spectroscopy, Smith degradation, chromium trioxide and enzymic degradations, in conjunction with methylation studies supported by fast-atom-bombardment mass spectrometry and linkage analyses by gas chromatography/mass spectrometry. It was concluded on the basis of the following structures, in which each was linked 1-->5 to a terminal 3-deoxy-D-manno-octulosonic acid residue, that the core regions with qualitatively similar sugar compositions showed serotypic differences in one or more of their sequences, linkage types, and anomeric configurations: [formula: see text] [corrected]. The outer regions of each structure carry Neu5Ac residues linked 2-->3 to available beta-D-Galp residues and show striking similarities with various glycosphingolipids of the ganglioside family. However, Neu5Ac epitopes are not apparently involved in determining serospecificity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Campylobacter jejuni / chemistry*
  • Campylobacter jejuni / classification
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Lipopolysaccharides / chemistry*
  • Molecular Sequence Data
  • N-Acetylneuraminic Acid
  • Neuraminidase / pharmacology
  • Oligosaccharides / chemistry
  • Serotyping
  • Sialic Acids / analysis


  • Lipopolysaccharides
  • Oligosaccharides
  • Sialic Acids
  • Neuraminidase
  • N-Acetylneuraminic Acid