Allergens of mammalian origin V. Properties of extracts derived from the domestic cat

Clin Allergy. 1976 Sep;6(5):419-28. doi: 10.1111/j.1365-2222.1976.tb01926.x.

Abstract

Eight commercial cat dander extracts and two pelt extracts derived from mongrel and Siamese cats were compared. Cat allergn 1 and cat albumin were measured by radial immunodiffusion. Allergenic activity was evaluated by prick test and a modified radioallergosorbent test. In the latter, the dilution of each extract that produced 50% inhibition of binding of IgE antibodies to insolubilized cat allergen 1 (RAST 1) and insolubilized cat serum (RAST 2) was determind. The total non-dialysable solid content of the extracts did not correlate with any other parameter. Cat allergen 1 content determined by radial immunodiffusion correlated with average prick test results in ten cat-sensitive subjects and with RAST 1 activity. Cat albumin content correlated weakly with RAST 2 activity but not with any other measure of allergenic activity. Absorption of each extract with the gamma-globulin fraction of rabbit antiserum to cat allergen 1 significantly reduced prick test reactivity and RAST 1 activity, but not RAST 2 activity. These results indicate that cat allergen 1 is an important allergen in cat dander extracts and its measurement may be used to standardize the allergenic activity of such extracts.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Albumins / immunology
  • Allergens / isolation & purification*
  • Animals
  • Antibodies
  • Antigens
  • Cats / immunology*
  • Epithelium / immunology
  • Hair / immunology*
  • Humans
  • Immunodiffusion
  • Rabbits / immunology
  • Radioallergosorbent Test
  • Skin Tests

Substances

  • Albumins
  • Allergens
  • Antibodies
  • Antigens