Cloning and pharmacological characterization of a rat mu opioid receptor

Neuron. 1993 Nov;11(5):903-13. doi: 10.1016/0896-6273(93)90120-g.


We have isolated a rat cDNA clone that displays 75% amino acid homology with the mouse delta and rat kappa opioid receptors. The cDNA (designated pRMuR-12) encodes a protein of 398 amino acids comprising, in part, seven hydrophobic domains similar to those described for other G protein-linked receptors. Data from binding assays conducted with COS-1 cells transiently transfected with a CMV mammalian expression vector containing the full coding region of pRMuR-12 demonstrated mu receptor selectivity. In situ hybridization mRNA analysis revealed an mRNA distribution in rat brain that corresponds well to the distribution of binding sites labeled with mu-selective ligands. Based upon these observations, we conclude that pRMuR-12 encodes a mu opioid receptor.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Cell Line, Transformed
  • Cloning, Molecular*
  • DNA / genetics
  • DNA, Complementary / genetics
  • Male
  • Molecular Sequence Data
  • Oligonucleotide Probes / genetics
  • Protein Conformation
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Opioid, mu / genetics*
  • Receptors, Opioid, mu / metabolism*
  • Transfection


  • DNA, Complementary
  • Oligonucleotide Probes
  • RNA, Messenger
  • Receptors, Opioid, mu
  • DNA

Associated data

  • GENBANK/L22455