Phylogeny of twenty Thermus isolates constructed from 16S rRNA gene sequence data

Int J Syst Bacteriol. 1993 Oct;43(4):754-60. doi: 10.1099/00207713-43-4-754.

Abstract

The sequences of the 16S rRNA genes of 20 Thermus isolates were determined to a high fidelity by using automated DNA sequencing and fluorescent-dye-labelled primers. The strains tested included members of the three validly named Thermus species and representatives of major taxonomic clusters defined previously for this genus. The parsimony method was used to reconstruct the phylogeny of the strains from the aligned sequences, and a bootstrap analysis revealed a number of well-supported clades. Our results are not consistent with groupings inferred from numerical taxonomy data but support the conjecture that the genus Thermus contains more species than the three currently recognized species.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • Consensus Sequence
  • DNA Primers
  • DNA, Ribosomal / genetics*
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 16S / genetics*
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Thermus / classification*
  • Thermus / genetics*

Substances

  • DNA Primers
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S