Molecular cloning and chromosomal assignment of the gene for human Zn-alpha 2-glycoprotein

Biochemistry. 1993 Dec 7;32(48):12968-76. doi: 10.1021/bi00211a004.

Abstract

Genomic clones containing the human Zn-alpha 2-glycoprotein gene were isolated. Two of them were for the functional gene overlapped, and the other four were for two different pseudogenes (1 and 2) retaining exon-intron organization. The complete DNA sequence of the functional gene (9.3 kb) and its 5'- and 3'-flanking regions (5.3 and 0.1 kb, respectively) was determined. The gene is composed of four exons; the first exon is for the 5'-untranslated region, the signal sequence, and the first six amino acids; the second, for domain A; the third, for domain B; and the fourth, for domain C and the 3'-untranslated region. The 5'-flanking region contains a TATA box, a CAT box, an octamer sequence, and three possible Spl-binding sites. Ten and three copies of Alu repetitive DNA were identified within the gene and the 5'-flanking region, respectively, and they occupy 30% of the gene. The nucleotide sequences around the exons of pseudogene 1 were also determined; they had high homology (90-91%) with the corresponding region of the functional gene. Southern blot analysis suggested that there are only three genes, including nonfunctional ones, for Zn-alpha 2-glycoprotein in humans. The gene (ZA2G) was mapped to human chromosome band 7q22.1 by fluorescence in situ hybridization.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Human, Pair 7*
  • Cloning, Molecular
  • Genes
  • Genomic Library
  • Glycoproteins / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Molecular Sequence Data
  • Pseudogenes
  • Seminal Plasma Proteins*
  • Sequence Alignment
  • Sequence Homology, Nucleic Acid

Substances

  • Glycoproteins
  • Seminal Plasma Proteins
  • Zn-alpha-2-glycoprotein

Associated data

  • GENBANK/D14034
  • GENBANK/D17642
  • GENBANK/D17799
  • GENBANK/D17800
  • GENBANK/D17801
  • GENBANK/D17802
  • GENBANK/S56557
  • GENBANK/X71481
  • GENBANK/X71774
  • GENBANK/X74555