Cloning of a TGF beta type I receptor that forms a heteromeric complex with the TGF beta type II receptor

Cell. 1993 Nov 19;75(4):681-92. doi: 10.1016/0092-8674(93)90489-d.

Abstract

A cDNA clone encoding a 53 kd serine/threonine kinase receptor with an overall structure similar to that of the type II receptor for transforming growth factor beta (TGF beta) was obtained. 125I-TGF beta 1 bound to porcine endothelial cells transfected with the cDNA and formed a cross-linked complex of 70 kd, characteristic of a TGF beta type I receptor. Immunoprecipitation of the cross-linked complexes by antibodies against the cloned receptor revealed the 70 kd complex as well as a 94 kd TGF beta type II receptor complex. The immunoprecipitated novel serine/threonine kinase receptor had biochemical properties of the TGF beta type I receptor and was observed in different cell types. Transfection of the cloned cDNA into TGF beta type I receptor-deficient cells restored TGF beta-induced plasminogen activator inhibitor 1 production. These results suggest that signal transduction by TGF beta involves the formation of a heteromeric complex of two different serine/threonine kinase receptors.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cells, Cultured
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Gene Expression
  • Humans
  • In Vitro Techniques
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / genetics
  • Molecular Sequence Data
  • Protein-Serine-Threonine Kinases / chemistry
  • Protein-Serine-Threonine Kinases / genetics*
  • RNA, Messenger / genetics
  • Receptors, Transforming Growth Factor beta / chemistry
  • Receptors, Transforming Growth Factor beta / genetics*
  • Receptors, Transforming Growth Factor beta / metabolism
  • Recombinant Proteins / metabolism
  • Restriction Mapping
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Swine
  • Tissue Distribution
  • Transfection
  • Transforming Growth Factor beta / metabolism

Substances

  • DNA, Complementary
  • Membrane Glycoproteins
  • RNA, Messenger
  • Receptors, Transforming Growth Factor beta
  • Recombinant Proteins
  • Transforming Growth Factor beta
  • Protein-Serine-Threonine Kinases

Associated data

  • GENBANK/L11695
  • GENBANK/L20888
  • GENBANK/L20889
  • GENBANK/L22499
  • GENBANK/L26083
  • GENBANK/L33709
  • GENBANK/S60904
  • GENBANK/S60905
  • GENBANK/S60924
  • GENBANK/U00968