Impaired gene transcription and nuclear protein kinase C activation in the brain and liver of aged rats

FEBS Lett. 1993 Nov 22;334(3):351-4. doi: 10.1016/0014-5793(93)80710-c.


The expression of the hsp70 and c-fos genes and the activation of nuclear protein kinase C (PKC) were studied in young and aged whole rats under heat-shock conditions. The induction of hsp70 and c-fos genes by heat shock were decreased several fold in the brain as well as in the liver of senescent animals. Nuclear run-off transcription assay indicated that this age-related impairment could be attributed to a block at the level of transcription. Nuclear PKC activation by heat shock was not apparent in old animals. Nuclear PKC involvement in the repression of transcription during senescence is postulated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / genetics
  • Aging / metabolism*
  • Animals
  • Brain / enzymology
  • Brain / metabolism*
  • Cell Nucleus / enzymology
  • Enzyme Activation
  • Heat-Shock Proteins / genetics
  • Liver / enzymology
  • Liver / metabolism*
  • Male
  • Protein Kinase C / metabolism*
  • Proto-Oncogene Proteins c-fos / genetics
  • Rats
  • Rats, Wistar
  • Transcription, Genetic*


  • Heat-Shock Proteins
  • Proto-Oncogene Proteins c-fos
  • Protein Kinase C