A Saccharomyces cerevisiae protein-serine kinase related to mammalian glycogen synthase kinase-3 and the Drosophila melanogaster gene shaggy product

Gene. 1993 Nov 30;134(1):51-6. doi: 10.1016/0378-1119(93)90173-z.


The glycogen synthase kinase-3 (GSK-3) family of protein-serine kinases is implicated in the development and hormonal regulation of higher eukaryotes. GSK-3-related genes have been cloned and characterized in mammals (alpha and beta forms), Drosophila melanogaster (shaggy/zeste-white3) and Saccharomyces cerevisiae (MCK1). Using the polymerase chain reaction and primers designed to hybridize to conserved catalytic domain sequences of this family, a genomic fragment was amplified from budding yeast DNA. Genomic clones encompassing the entire reading frame were subsequently isolated and sequenced. The protein encoded by this gene, termed ScGSK-3, displays high identity with members of the GSK-3 family, sharing several structural features including a regulatory Tyr residue. A phylogenetic analysis of the catalytic domains of these protein kinases suggests that ScGSK-3 represents the bona fide homologue of GSK-3 and the shaggy product, while the related MCK1 protein kinase is encoded by a paralogous gene which originated by a gene duplication event in the yeast lineage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Calcium-Calmodulin-Dependent Protein Kinases / genetics*
  • DNA
  • Drosophila Proteins*
  • Drosophila melanogaster / genetics*
  • Glycogen Synthase Kinase 3
  • Glycogen Synthase Kinases
  • Microtubule-Associated Proteins / genetics*
  • Molecular Sequence Data
  • Phylogeny
  • Protein Kinases / genetics*
  • Protein-Serine-Threonine Kinases / genetics*
  • Saccharomyces cerevisiae / enzymology*
  • Sequence Homology, Amino Acid


  • Drosophila Proteins
  • Microtubule-Associated Proteins
  • DNA
  • Protein Kinases
  • Glycogen Synthase Kinases
  • Protein-Serine-Threonine Kinases
  • Sgg protein, Drosophila
  • Calcium-Calmodulin-Dependent Protein Kinases
  • Glycogen Synthase Kinase 3

Associated data

  • GENBANK/L12761
  • GENBANK/L24529
  • GENBANK/X72887
  • GENBANK/X72985
  • GENBANK/X72986
  • GENBANK/X72987
  • GENBANK/X72988
  • GENBANK/X72989
  • GENBANK/Z15047
  • GENBANK/Z15048