Cervical sampling for diagnosis of genital chlamydial infection with a new brush device

Genitourin Med. 1993 Oct;69(5):397-9. doi: 10.1136/sti.69.5.397.

Abstract

Objectives: To compare a new sampling device, a brush, Accellone-Multi-Instrument (AMI), with a dacron-tipped swab for detection of Chlamydia trachomatis in endocervical specimens, and to evaluate if consecutive multiple cervical sampling as compared with such a single specimen would increase the sensitivity.

Methods: 501 females attending an STD clinic and 172 females attending a family planning clinic were examined prospectively. Two cervical specimens were collected from each woman. C trachomatis were detected by culture or enzyme immunoassay (IDEIA-III). Positive EIA samples were confirmed by a direct immunofluorescent test.

Results: When cervical specimens were collected with the brush as the first device, 92% of the culture-positive cases were detected, and when the samples were collected with the dacron-tipped-swab first, 84% of the culture-positive cases were detected (p < 0.05). The first collected specimen detected 89% of the culture-positive cases and 81% of those that were positive by IDEIA.

Conclusions: The study indicates that the AMI brush is superior to non-toxic, dacron-tipped swabs for detection of C trachomatis in cervical samples by cell culture but not by ELISA, and that the sensitivity could be improved by analysing multiple cervical samples.

Publication types

  • Comparative Study

MeSH terms

  • Adolescent
  • Adult
  • Chlamydia Infections / diagnosis*
  • Chlamydia trachomatis / isolation & purification
  • Female
  • Gynecology / instrumentation*
  • Humans
  • Immunoenzyme Techniques
  • Prospective Studies
  • Specimen Handling / instrumentation
  • Uterine Cervical Diseases / diagnosis*
  • Uterine Cervical Diseases / microbiology