Analysis of T cell receptor V beta regions expressed by rheumatoid synovial T lymphocytes

Immunobiology. 1993 Aug;188(4-5):330-9. doi: 10.1016/s0171-2985(11)80217-2.


The T cell receptor (TCR) V beta gene segment repertoire of T lymphocytes derived from peripheral blood of two healthy individuals and synovial tissue, synovial fluid and peripheral blood of three rheumatoid arthritis (RA) patients was analyzed. A sensitive assay based on the amplification of cDNA by the polymerase chain reaction (PCR) was used to analyze the levels of expression of 20 TCR V beta gene segment families. The relative expression of V beta gene segments in lymphocytes derived from peripheral blood, synovial tissue and synovial fluid was conserved over 155 days in one patient. V beta 9 transcripts were undetectable in the cells of this individual. In the two other patients the frequency of V beta 2 transcripts in synovial T cells of affected joints was significantly higher than in their peripheral blood lymphocytes. Dominance of distinct rearrangements among the V beta 2 transcripts from the synovial cells of these patients support the idea that the synovial T cell response is driven by antigen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arthritis, Rheumatoid / genetics
  • Arthritis, Rheumatoid / immunology*
  • Base Sequence
  • DNA Primers / genetics
  • DNA, Complementary / genetics
  • Gene Expression
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Receptors, Antigen, T-Cell, alpha-beta / genetics*
  • Synovial Fluid / immunology
  • Synovial Membrane / immunology
  • T-Lymphocytes / immunology*
  • Tissue Distribution
  • Transcription, Genetic


  • DNA Primers
  • DNA, Complementary
  • Receptors, Antigen, T-Cell, alpha-beta