Expression of the baculovirus p35 gene inhibits mammalian neural cell death

J Neurochem. 1993 Dec;61(6):2318-21. doi: 10.1111/j.1471-4159.1993.tb07477.x.

Abstract

Expression of the apoptosis suppressor gene p35, derived from the baculovirus Autographa californica nuclear polyhedrosis virus, markedly inhibited the cell death of stably transfected mammalian neural cells whether the cell death was induced by glucose withdrawal, calcium ionophore, or serum withdrawal. The p35 protein, which is required to block virus-induced apoptosis of cultured insect cells, is only the second gene product shown to block mammalian neural cell death, with Bcl-2 being the first. Because there is no apparent homology between p35 and Bcl-2, the existence of a cellular death program that may be modulated at multiple points is suggested. Furthermore, these findings demonstrate that the putative cellular death program is conserved across species and cell types.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Apoptosis / physiology*
  • Cell Line
  • Gene Expression
  • Genes, Suppressor*
  • Genes, Viral*
  • Mesencephalon / cytology
  • Neurons / cytology*
  • Nucleopolyhedroviruses / genetics*
  • Protein-Tyrosine Kinases / biosynthesis
  • Proto-Oncogene Proteins / biosynthesis
  • Proto-Oncogene Proteins c-bcl-2
  • Rats
  • Substantia Nigra / cytology
  • Transfection
  • Viral Proteins / biosynthesis*

Substances

  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Viral Proteins
  • p35 protein, Nucleopolyhedrovirus
  • Protein-Tyrosine Kinases