Cholecystokinin-A but not cholecystokinin-B receptor stimulation induces endogenous opioid-dependent antinociceptive effects in the hot plate test in mice

Neurosci Lett. 1993 Oct 1;160(2):193-6. doi: 10.1016/0304-3940(93)90411-d.

Abstract

The effects of intracerebroventricular administration of the cholecystokinin (CCK) analogue, BDNL, and the selective CCK-B agonist, BC 264, were determined using the hot plate test in mice. BDNL (0.2 nmol and 0.5 nmol) increased the jump and the paw lick latencies. These effects were blocked by the CCK-A antagonist MK-329 (0.02 mg/kg), supporting the involvement of CCK-A receptors in CCK-induced analgesia. In contrast, the selective CCK-B agonist BC 264 produced, at one dose (2.5 nmol), a slight decrease in the lick latency that was only antagonized by the CCK-B antagonist. Naloxone, but not naltrindole, antagonized BDNL-induced analgesia. The results suggest that activation of CCK-A receptors by BDNL leads to antinociceptive responses indirectly mediated by stimulation of mu-opioid receptors by endogenous enkephalins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzodiazepinones / administration & dosage
  • Benzodiazepinones / pharmacology*
  • Cerebral Ventricles / drug effects
  • Cerebral Ventricles / physiology*
  • Cholecystokinin / administration & dosage
  • Cholecystokinin / analogs & derivatives*
  • Cholecystokinin / pharmacology
  • Devazepide
  • Hot Temperature
  • Injections, Intraventricular
  • Male
  • Mice
  • Mice, Inbred Strains
  • Naloxone / pharmacology
  • Pain / physiopathology*
  • Peptide Fragments / administration & dosage
  • Peptide Fragments / pharmacology
  • Phenylurea Compounds*
  • Receptors, Cholecystokinin / antagonists & inhibitors
  • Receptors, Cholecystokinin / physiology*
  • Sincalide / administration & dosage
  • Sincalide / analogs & derivatives
  • Sincalide / pharmacology

Substances

  • BC 264
  • Benzodiazepinones
  • Peptide Fragments
  • Phenylurea Compounds
  • Receptors, Cholecystokinin
  • Naloxone
  • L 365260
  • Cholecystokinin
  • cholecystokinin (27-33), tert-butyloxycarbonyl-Nle(28,31)-
  • Devazepide
  • Sincalide