Gastric DNA-binding proteins recognize upstream sequence motifs of parietal cell-specific genes

Proc Natl Acad Sci U S A. 1993 Nov 15;90(22):10876-80. doi: 10.1073/pnas.90.22.10876.

Abstract

Polymerase chain reaction amplification of cDNA from pig gastric mucosa demonstrated the presence of zinc-finger proteins called GATA-GT1, GATA-GT2, and GATA-GT3, each having zinc-finger sequences similar to previously characterized GATA-binding proteins. Subsequently, full-length cDNAs of GATA-GT1 and GATA-GT2 were obtained from rat stomach. The zinc-finger domains of GATA-GT1 and -GT2 were 66-86% identical on the amino acid level with each other and with other GATA-binding proteins. Potential protein kinase phosphorylation sites were present in the zinc-finger region. In contrast, regions outside the zinc fingers shared significantly lower similarities. GATA-GT2 was found to bind to the upstream sequence of the H+/K(+)-ATPase beta gene and to a sequence containing the GATA motif. GATA-GT1 and -GT2 were expressed predominantly in the gastric mucosa and at much lower levels in the intestine (GATA-GT2, also in testis), their tissue distributions being distinct from those of GATA-1, -2, or -3. These results clearly suggest that GATA-GT1 and GATA-GT2 are involved in gene regulation specifically in the gastric epithelium and represent two additional members of the GATA-binding protein family.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers / chemistry
  • DNA, Complementary / genetics
  • DNA-Binding Proteins / genetics*
  • GATA4 Transcription Factor
  • GATA6 Transcription Factor
  • Gastric Mucosa / physiology*
  • Gene Expression
  • Molecular Sequence Data
  • Multigene Family
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics
  • Rats
  • Rats, Sprague-Dawley
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Swine
  • Tissue Distribution
  • Transcription Factors / genetics*
  • Zinc Fingers*

Substances

  • DNA Primers
  • DNA, Complementary
  • DNA-Binding Proteins
  • GATA4 Transcription Factor
  • GATA6 Transcription Factor
  • Gata4 protein, rat
  • Gata6 protein, rat
  • RNA, Messenger
  • Transcription Factors

Associated data

  • GENBANK/L22760
  • GENBANK/L22761