Efficient gene transfer with less cytotoxicity by means of cationic multilamellar liposomes

Biochem Biophys Res Commun. 1993 Nov 15;196(3):1042-8. doi: 10.1006/bbrc.1993.2356.


A simple procedure for the preparation of cationic multilamellar vesicles (MLV) consisting of N-(alpha-trimethylammonioacetyl)-didodecyl-D-glutamate chloride, dilauroyl phosphatidylcholine, and dioleoyl phosphatidylethanolamine in a molar ratio of 1:2:2 was devised. When bacteriophage lambda DNA was encapsulated into these liposomes, entrapment efficiency was found to be nearly 100%, and digestibility of the DNA was less than 10%. Upon encapsulation of the plasmid pCH110 into cationic MLV, efficient expression was comparable to that obtained with cationic vesicles prepared by reverse-phase evaporation method (REV). Cytotoxicity of the present liposomes was less than that of cationic REV and far less than that of Lipofectin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bacteriophage lambda / genetics*
  • Cell Line
  • Cell Survival*
  • Chlorocebus aethiops
  • Cloning, Molecular
  • DNA, Viral / administration & dosage
  • DNA, Viral / metabolism*
  • Drug Carriers
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Gene Transfer Techniques*
  • Genes, Bacterial
  • Glutamates
  • Liposomes
  • Pentosyltransferases / biosynthesis*
  • Pentosyltransferases / genetics
  • Phosphatidylcholines
  • Phosphatidylethanolamines
  • Plasmids*


  • DNA, Viral
  • Drug Carriers
  • Glutamates
  • Liposomes
  • Phosphatidylcholines
  • Phosphatidylethanolamines
  • didodecyl N-(alpha-trimethylammonioacetyl)glutamate
  • 1,2-dilauroylphosphatidylcholine
  • 1,2-dielaidoylphosphatidylethanolamine
  • Pentosyltransferases
  • xanthine phosphoribosyltransferase