Structural characterization of feruloyl oligosaccharides from spinach-leaf cell walls

Carbohydr Res. 1993 Oct 4;248:179-90. doi: 10.1016/0008-6215(93)84125-p.

Abstract

Hydrolysis of spinach-leaf cell walls with Driselase (a fungal enzyme preparation) released two arabino-oligosaccharides and one galactobiose, each carrying a ferulic acid moiety. The oligosaccharides were characterized by NMR spectroscopy, methylation analysis, and FABMS. They were O-(2-O-trans-feruloyl-alpha-L-arabinofuranosyl)-(1-->5)-L-arabinof uranose, O-(6-O-trans-feruloyl-beta-D-galactopyranosyl)-(1-->4)-D-galactopy ranose, and O-alpha-L-arabinofuranosyl-(1-->3)-O-(2-O-trans-feruloyl-alpha-L-a rabino- furanosyl)-(1-->5)-L-arabinofuranose.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabinose / analysis
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Cell Wall / chemistry
  • Coumaric Acids / chemistry*
  • Fungal Proteins*
  • Galactose
  • Glycoside Hydrolases
  • Magnetic Resonance Spectroscopy
  • Methylation
  • Molecular Sequence Data
  • Molecular Structure
  • Oligosaccharides / chemistry*
  • Oligosaccharides / isolation & purification
  • Spectrometry, Mass, Fast Atom Bombardment
  • Vegetables / chemistry*

Substances

  • Coumaric Acids
  • Fungal Proteins
  • Oligosaccharides
  • ferulic acid
  • Arabinose
  • Glycoside Hydrolases
  • driselase
  • Galactose