The C. Elegans Heterochronic Gene lin-4 Encodes Small RNAs With Antisense Complementarity to lin-14

Cell. 1993 Dec 3;75(5):843-54. doi: 10.1016/0092-8674(93)90529-y.

Abstract

lin-4 is essential for the normal temporal control of diverse postembryonic developmental events in C. elegans. lin-4 acts by negatively regulating the level of LIN-14 protein, creating a temporal decrease in LIN-14 protein starting in the first larval stage (L1). We have cloned the C. elegans lin-4 locus by chromosomal walking and transformation rescue. We used the C. elegans clone to isolate the gene from three other Caenorhabditis species; all four Caenorhabditis clones functionally rescue the lin-4 null allele of C. elegans. Comparison of the lin-4 genomic sequence from these four species and site-directed mutagenesis of potential open reading frames indicated that lin-4 does not encode a protein. Two small lin-4 transcripts of approximately 22 and 61 nt were identified in C. elegans and found to contain sequences complementary to a repeated sequence element in the 3' untranslated region (UTR) of lin-14 mRNA, suggesting that lin-4 regulates lin-14 translation via an antisense RNA-RNA interaction.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Caenorhabditis / embryology
  • Caenorhabditis / genetics*
  • DNA Primers / chemistry
  • Gene Expression Regulation
  • Genes, Helminth*
  • Helminth Proteins / genetics*
  • Hydrogen Bonding
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Phylogeny
  • Protein Biosynthesis
  • RNA, Antisense*
  • RNA, Messenger / genetics
  • Sequence Alignment
  • Sequence Homology, Nucleic Acid
  • Time Factors

Substances

  • DNA Primers
  • Helminth Proteins
  • RNA, Antisense
  • RNA, Messenger

Associated data

  • GENBANK/L20888
  • GENBANK/L20889
  • GENBANK/L33709
  • GENBANK/S60904
  • GENBANK/S60905
  • GENBANK/S60924
  • GENBANK/U01829
  • GENBANK/U01830
  • GENBANK/U01837
  • GENBANK/U01838