Isolation and characterization of novel genes which are expressed during the very early stage of zygote formation in Chlamydomonas reinhardtii

Curr Genet. 1993 Oct;24(4):296-300. doi: 10.1007/BF00336779.

Abstract

We have obtained five cDNA clones which are complementary to transcripts that accumulate specifically during the early stage of zygote formation in Chlamydomonas reinhardtii. Based on the time-course of transcript accumulation of their corresponding genes, these clones were classified into two groups: an early expression group (three clones; pZS102-1, pZS102-69, pZS1802) and a late expression group (two clones; pZS1860 and pZS1886). The levels of transcripts in the former group reached a maximum 30 min after the onset of mating, whereas those in the latter group peaked at 3 h. The addition of 10 micrograms/ml of cycloheximide reduced the decline in the levels of transcripts, except in the transcript corresponding to pZS102-69. Two clones, pZS102-1 and pZS102-69, contained highly-homologous predicted open-reading frames, and were judged to be cDNAs for two members of a novel gene family. These genes were referred to as zys1A and zys1B, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Chlamydomonas reinhardtii / genetics*
  • Chlamydomonas reinhardtii / growth & development
  • DNA
  • Molecular Sequence Data
  • Multigene Family
  • Transcription, Genetic
  • Zygote / metabolism*

Substances

  • DNA

Associated data

  • GENBANK/L08130
  • GENBANK/L08131
  • GENBANK/X59683
  • GENBANK/X71676
  • GENBANK/X71677
  • GENBANK/X71678
  • GENBANK/X71679
  • GENBANK/X71680
  • GENBANK/X76117
  • GENBANK/Z26341