Autocatalytic modification of human carbonyl reductase by 2-oxocarboxylic acids

FEBS Lett. 1993 Dec 6;335(2):151-4. doi: 10.1016/0014-5793(93)80719-b.


Carbonyl reductase occurs in multiple molecular forms. Sequence analysis has yielded a carboxyethyllysine residue in one of the enzyme forms, suggesting that pyruvate has been incorporated in a posttranslational enzymatic reaction [Krook, M., Ghosh, D., Strömberg R., Carlquist, M. and Jörnvall, H. (1993) Proc. Natl. Acad. Sci. USA 90, 502-506]. Using highly purified carbonyl reductase from human brain we show that pyruvate and other 2-oxocarboxylic acids are bound to the enzyme in an autocatalytic reaction. The resulting enzyme forms were indistinguishable from the native enzyme forms by electrophoresis and isoelectric focusing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Oxidoreductases / chemistry*
  • Brain / enzymology
  • Carboxylic Acids / chemistry*
  • Catalysis
  • Humans
  • Keto Acids / chemistry
  • Pyruvates / chemistry
  • Pyruvic Acid


  • Carboxylic Acids
  • Keto Acids
  • Pyruvates
  • Pyruvic Acid
  • Alcohol Oxidoreductases