Long-term Persistent Infection of Swine monocytes/macrophages With African Swine Fever Virus

J Virol. 1994 Jan;68(1):580-3.

Abstract

Long-term persistent infection was established in 100% of pigs (n = 19) experimentally infected with African swine fever virus (ASFV). Viral DNA was detected in peripheral blood mononuclear leukocytes (PBML) at greater than 500 days postinfection by a PCR assay. Infectious virus was not, however, isolated from the same PBML samples. In cell fractionation studies of PBML, monocytes/macrophages were found to harbor viral DNA during the persistent phase of infection. This result indicates that monocytes/macrophages are persistently infected with ASFV and that ASFV-swine monocyte/macrophage interactions can result in either lytic or persistent infection.

MeSH terms

  • African Swine Fever / microbiology*
  • African Swine Fever Virus / genetics
  • African Swine Fever Virus / isolation & purification*
  • Animals
  • Base Sequence
  • Capsid / genetics
  • Capsid Proteins*
  • DNA, Viral / blood*
  • Genes, Viral
  • Macrophages / microbiology*
  • Molecular Sequence Data
  • Monocytes / microbiology*
  • Polymerase Chain Reaction
  • Swine
  • Time Factors

Substances

  • Capsid Proteins
  • DNA, Viral
  • capsid protein p72, African swine fever virus