Characterization of a human chromosome 4 flow-sorted cosmid library

Cytogenet Cell Genet. 1994;65(4):238-42. doi: 10.1159/000133638.

Abstract

A cosmid library has been constructed from a hamster x human hybrid cell line and gridded into 270 microtiter plates containing a total of 25,920 single colonies. Approximately 84% of the recombinants contain human material, with an average length of 29 kb. This library represents a nearly three-fold coverage of human chromosome 4. We investigated this library for presumptive genes, using a set of oligonucleotides detecting SpI and splice-site consensus sequences. The presence of simple repeat motifs was investigated in the cosmids using the oligonucleotides (GGATTT)3, (GGAT)4, (CAC)5, (GCC)5, (AGC)5, (GATA)4, (GACA)4, and (CA)8 as hybridization probes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Chromosomes, Human, Pair 4*
  • Cloning, Molecular
  • Consensus Sequence
  • Cosmids*
  • Cricetinae
  • Flow Cytometry
  • Gene Library*
  • Humans
  • Hybrid Cells
  • Molecular Sequence Data
  • RNA Splicing
  • Repetitive Sequences, Nucleic Acid