Isotype-specific regulation of human lymphocyte production of immunoglobulins by sustained exposure to vasoactive intestinal peptide

J Allergy Clin Immunol. 1993 Dec;92(6):891-901. doi: 10.1016/0091-6749(93)90067-p.

Abstract

Exposure of lymphocytes to nanomolar to micromolar concentrations of vasoactive intestinal peptide (VIP) for 1 to 3 days only modestly suppressed or enhanced the production of IgA and IgM, but not IgG. The effects of twice daily additions of 10(-12) to 10(-7) mol/L VIP for up to 18 days on pokeweed mitogen-stimulated peripheral blood mononuclear cells (PBMCs) from normal human subjects was examined by quantifying the production of IgG, IgM, and IgA. The maximum suppression of IgG by 10(-9) mol/L VIP was 79% +/- 33% (mean +/- SD) (range, 41% to 97%; p < 0.015) on day 9 and 84% +/- 1% (range, 74% to 96%; p < 0.0001) on day 14 and was significant at 6 x 10(-10) to 4 x 10(-9) mol/L VIP. Suppression of IgM production by 10(-9) mol/L VIP was significant and was observed first on day 5 and persisted through day 14. VIP did not alter IgA production or affect the proliferation or viability of PBMCs. The production of IgE by interleukin-4 stimulated PBMCs was enhanced consistently in two subjects but not in two other subjects. The duration of exposure to nanomolar concentrations of VIP is thus a critical determinant of its immunoregulatory effect, as manifested by late suppression of production of IgG and IgM and concurrent enhancement of production of IgE in some subjects.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • B-Lymphocyte Subsets / cytology
  • B-Lymphocyte Subsets / drug effects
  • B-Lymphocyte Subsets / immunology
  • Cell Division / drug effects
  • Humans
  • Immunoglobulin A / biosynthesis
  • Immunoglobulin E / biosynthesis
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin Isotypes / biosynthesis*
  • Immunoglobulin M / biosynthesis
  • In Vitro Techniques
  • Interleukin-4 / pharmacology
  • Lymphocytes / cytology
  • Lymphocytes / drug effects*
  • Lymphocytes / immunology*
  • T-Lymphocyte Subsets / cytology
  • T-Lymphocyte Subsets / drug effects
  • T-Lymphocyte Subsets / immunology
  • Vasoactive Intestinal Peptide / pharmacology*

Substances

  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin Isotypes
  • Immunoglobulin M
  • Interleukin-4
  • Vasoactive Intestinal Peptide
  • Immunoglobulin E