Association of the APC gene product with beta-catenin

Science. 1993 Dec 10;262(5140):1731-4. doi: 10.1126/science.8259518.


Mutations in the human APC gene are linked to familial adenomatous polyposis and to the progression of sporadic colorectal and gastric tumors. To gain insight into APC function, APC-associated proteins were identified by immunoprecipitation experiments. Antibodies to APC precipitated a 95-kilodalton protein that was purified and identified by sequencing as beta-catenin, a protein that binds to the cell adhesion molecule E-cadherin. An antibody specific to beta-catenin also recognized the 95-kilodalton protein in the immunoprecipitates. These results suggest that APC is involved in cell adhesion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenomatous Polyposis Coli Protein
  • Amino Acid Sequence
  • Antibodies
  • Cadherins / metabolism*
  • Cell Adhesion
  • Cell Line
  • Colonic Neoplasms / genetics
  • Colonic Neoplasms / metabolism*
  • Cytoskeletal Proteins / chemistry
  • Cytoskeletal Proteins / isolation & purification
  • Cytoskeletal Proteins / metabolism*
  • Genes, APC*
  • Humans
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics
  • Neoplasm Proteins / immunology
  • Neoplasm Proteins / metabolism*
  • Precipitin Tests
  • Trans-Activators*
  • Tumor Cells, Cultured
  • beta Catenin


  • Adenomatous Polyposis Coli Protein
  • Antibodies
  • CTNNB1 protein, human
  • Cadherins
  • Cytoskeletal Proteins
  • Neoplasm Proteins
  • Trans-Activators
  • beta Catenin