Tracing B cell development in human germinal centres by molecular analysis of single cells picked from histological sections

EMBO J. 1993 Dec 15;12(13):4955-67.

Abstract

Germinal centres are areas of intense B lymphocyte proliferation inside primary B cell follicles in spleen and lymph nodes. Rearranged V genes from single human B cells, isolated from histological sections of two such structures by micromanipulation, were amplified and sequenced. Cells from the follicular mantle were clonally diverse and largely expressed germline V genes. Germinal centres were dominated by a few large B cell clones dispersed throughout these structures and exhibiting intraclonal diversity by ongoing somatic hypermutation. Pronounced counterselection of replacement mutations seen in one of the germinal centres may indicate a late phase of the germinal centre reaction. A polyclonal population of activated B cells expressing unmutated antibodies in the dark zone of the other germinal centre may represent the initial founder cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Amino Acid Sequence
  • B-Lymphocytes / cytology*
  • Base Sequence
  • Cell Differentiation
  • Child
  • DNA Primers / chemistry
  • Gene Rearrangement, B-Lymphocyte*
  • Genes, Immunoglobulin*
  • Humans
  • Immunoglobulin Heavy Chains / genetics
  • Immunoglobulin Variable Region / genetics
  • Lymph Nodes / cytology*
  • Micromanipulation
  • Molecular Sequence Data
  • Mutation
  • Sequence Alignment
  • Sequence Homology, Nucleic Acid
  • Spleen / cytology*

Substances

  • DNA Primers
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Variable Region

Associated data

  • GENBANK/X73160
  • GENBANK/X73161
  • GENBANK/X73162
  • GENBANK/X73163
  • GENBANK/X73164
  • GENBANK/X73165
  • GENBANK/X73166
  • GENBANK/X73167
  • GENBANK/X73168
  • GENBANK/X73169
  • GENBANK/X73170
  • GENBANK/X73171
  • GENBANK/X73172
  • GENBANK/X73173
  • GENBANK/X73174
  • GENBANK/X73175
  • GENBANK/X73176
  • GENBANK/X73177
  • GENBANK/X73178
  • GENBANK/X73179
  • GENBANK/X73180
  • GENBANK/X73181
  • GENBANK/X73182
  • GENBANK/X73183
  • GENBANK/X73184
  • GENBANK/X73185
  • GENBANK/X73186
  • GENBANK/X73187
  • GENBANK/X73188
  • GENBANK/X73189