Molecular cloning of a novel putative G protein-coupled receptor (GPCR21) which is expressed predominantly in mouse central nervous system

FEBS Lett. 1993 Dec 27;336(2):317-22. doi: 10.1016/0014-5793(93)80828-i.


A novel cDNA clone encoding a putative G protein-coupled receptor (named GPCR21) was isolated from a mouse brain cDNA library along with its homologue, GPCR01 (the mouse counterpart of previously reported rat receptor R334 [(1991) FEBS Lett. 292, 243-248]) by the polymerase chain reaction using degenerate oligonucleotide primers. Northern blotting and reverse transcription-polymerase chain reaction analyses showed predominant expression of these two receptors in the central nervous system. In situ hybridization analysis revealed their prominent expression in the limbic system and further demonstrated the differential distribution of their mRNAs in mouse brain. Although the ligands for these receptors are yet to be identified, the significant sequence homology between these receptors suggests that they constitute a new receptor subfamily and they possibly represent different receptor subtypes for an unknown neurotransmitter.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / metabolism*
  • Cloning, Molecular
  • DNA, Complementary
  • GTP-Binding Proteins / metabolism*
  • Humans
  • Mice
  • Molecular Sequence Data
  • Receptors, Cell Surface / genetics*
  • Receptors, Cell Surface / metabolism
  • Receptors, G-Protein-Coupled*
  • Sequence Homology, Amino Acid


  • DNA, Complementary
  • Gpr12 protein, mouse
  • Receptors, Cell Surface
  • Receptors, G-Protein-Coupled
  • GTP-Binding Proteins

Associated data

  • GENBANK/D17292
  • GENBANK/D21061
  • GENBANK/D21062