Cloning of a mouse cDNA encoding DNA polymerase delta: refinement of the homology boxes

Gene. 1993 Dec 8;134(2):191-200. doi: 10.1016/0378-1119(93)90093-i.


A mouse DNA polymerase delta (Pol delta)-encoding cDNA (pol delta) was isolated by PCR amplification and cDNA library screening. The sequenced cDNA has a length of 3386 bp and the open reading frame (ORF) encodes a protein of 1105 amino acids (aa) with an M(r) of 123,743. The aa identity to the proteins encoded by the corresponding cDNA from Bos taurus (93%) and Homo sapiens (92%) is very high. The identity to the Pol delta from Schizosaccharomyces pombe, Saccharomyces cerevisiae and Plasmodium falciparum is around 50%. An aa comparison between all available Pol delta sequences reveals several common structural motifs. Polyclonal antibodies raised against a 31-aa synthetic peptide deduced from the ORF specifically recognize Pol delta polymerases from human cells and calf thymus in an immunoblot.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Cattle
  • Cloning, Molecular
  • DNA Polymerase III
  • DNA, Complementary
  • DNA-Directed DNA Polymerase / genetics*
  • Humans
  • Immunoblotting
  • Mice
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Sequence Homology, Amino Acid


  • DNA, Complementary
  • DNA Polymerase III
  • DNA-Directed DNA Polymerase

Associated data

  • GENBANK/L24529
  • GENBANK/Z14102
  • GENBANK/Z14103
  • GENBANK/Z14104
  • GENBANK/Z14105
  • GENBANK/Z14106
  • GENBANK/Z14107
  • GENBANK/Z15047
  • GENBANK/Z15048
  • GENBANK/Z21848