Insulin-like growth factor-1 and its receptor mediate the autocrine proliferation of human ovarian carcinoma cell lines

Lab Invest. 1993 Dec;69(6):756-60.


Background: IGF-1 regulates the growth of diverse mammalian cell types including several human carcinoma cell lines. The IGF-1 receptor is a glycosylated heterodimer which, upon binding with IGF-1, undergoes tyrosine autophosphorylation. The autophosphorylation of the beta-receptor subunit is a strict requirement for its mitogenic properties.

Experimental design: In this study, we have assessed the role of the IGF-1 receptor in the proliferation of ovarian carcinoma cell lines in culture. Effects of anti-sense and sense oligodeoxynucleotides to IGF-1 receptor RNA were tested.

Results: The human ovarian carcinoma cell lines OVCAR-3 and CaOV-3 both grew autonomously in serum-free medium, and their growth was further stimulated by the addition of IGF-1. Incubation of cells with anti-sense oligodeoxynucleotides corresponding to the IGF-1 receptor RNA markedly inhibited cell proliferation both in serum-free medium and in the presence of IGF-1. The inhibition of cell growth by the oligodeoxynucleotides corresponded to a reduction in the amount of detectable phosphorylated IGF-1 receptor.

Conclusions: The findings indicate that IGF-1 and its specific receptor mediate the autocrine proliferation of human ovarian carcinoma cell lines.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Carcinoma / pathology*
  • Cell Division
  • Female
  • Humans
  • Insulin-Like Growth Factor I / physiology*
  • Mice
  • Molecular Sequence Data
  • Oligonucleotides, Antisense / chemistry
  • Ovarian Neoplasms / pathology*
  • Phosphorylation
  • Receptor, IGF Type 1 / physiology*
  • Signal Transduction
  • Tumor Cells, Cultured


  • Oligonucleotides, Antisense
  • Insulin-Like Growth Factor I
  • Receptor, IGF Type 1