Oligonucleotide probe determination of tetracycline-resistant bacteria isolated from catfish ponds

Mol Cell Probes. 1993 Oct;7(5):345-8. doi: 10.1006/mcpr.1993.1051.


Oligonucleotide probes for class A, B and C tetracycline resistance determinants were synthesized and tested individually and in combination for hybridization with Gram-negative isolates previously characterized by restriction fragment DNA probes. Similar hybridization patterns were observed with either probe type. Gram-negative catfish pond bacteria were characterized by these oligonucleotide probes. The most common bacteria were Plesiomonas shigelloides (47.7%), Aeromonas hydrophila (28.8%) and Citrobacter freundii (13.3%). The class A (46.4%) tetracycline resistance determinant was more prevalent than B (19.8%) and C (0.3%). Some tetracycline-resistant isolates of each species, including most A. hydrophila (62.1%) isolates, failed to hybridize with any of the probes.

MeSH terms

  • Animals
  • Bacterial Typing Techniques
  • Base Sequence
  • Catfishes
  • DNA, Bacterial / genetics
  • Fresh Water
  • Gram-Negative Bacteria / classification*
  • Gram-Negative Bacteria / drug effects
  • Gram-Negative Bacteria / genetics
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Oligonucleotide Probes*
  • Tetracycline Resistance / genetics*
  • Water Microbiology*


  • DNA, Bacterial
  • Oligonucleotide Probes