Characterization and chromosomal localization of the human proto-oncogene BMI-1

Hum Mol Genet. 1993 Oct;2(10):1597-603. doi: 10.1093/hmg/2.10.1597.


The proto-oncogene bmi-1 is frequently activated by Moloney murine leukemia proviral insertions in E mu-myc transgenic mice1,2. Using a mouse bmi-1 cDNA probe a transcript of 3.3 kb was detected on Northern blots of human Burkitt's lymphoma cell lines. We have isolated and sequenced cDNA clones from a human erythroleukemia cell line (K562) derived cDNA library, using different mouse bmi-1 cDNA fragments as a probe. Analysis of genomic BMI-1 sequences reveals a gene structure which is very similar to that of the mouse, consisting of at least 10 exons. The human cDNA is 3203 bp in length and shows 86% identity to the mouse nucleotide sequence. The open reading frame encodes a protein of 326 amino acids which shares 98% identity to the amino acid sequence of mouse bmi-1 protein. In vitro translation experiments show that human cDNA derived RNA translates into a protein with a mobility of 44-46 kD on SDS polyacrylamide gels. Fluorescence in situ hybridization (FISH) on metaphase chromosome spreads located the human BMI-1 gene to the short arm of chromosome 10 (10p13), a region known to be involved in translocations in various leukemias.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Burkitt Lymphoma / pathology
  • Cell Line
  • Chromosome Mapping
  • Chromosomes, Human, Pair 10*
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • DNA-Binding Proteins*
  • Drosophila Proteins*
  • Drosophila melanogaster / genetics
  • Genes*
  • Genes, Insect
  • Humans
  • In Situ Hybridization, Fluorescence
  • Leukemia, Erythroblastic, Acute / pathology
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Nuclear Proteins / genetics*
  • Phylogeny
  • Polycomb Repressive Complex 1
  • Proteins / genetics
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogenes*
  • Repressor Proteins*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Translocation, Genetic


  • BMI1 protein, human
  • Bmi1 protein, mouse
  • DNA, Complementary
  • DNA-Binding Proteins
  • Drosophila Proteins
  • MAS1 protein, human
  • Nuclear Proteins
  • Proteins
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins
  • Repressor Proteins
  • Psc protein, Drosophila
  • Polycomb Repressive Complex 1

Associated data

  • GENBANK/L13689