RNA editing of AMPA receptor subunit GluR-B: a base-paired intron-exon structure determines position and efficiency

Cell. 1993 Dec 31;75(7):1361-70. doi: 10.1016/0092-8674(93)90622-w.


A functionally critical position (Q/R site) of the AMPA receptor subunit GluR-B is controlled by RNA editing that operates in the nucleus, since in brain and clonal cell lines of neural origin, unspliced GluR-B transcripts occur edited in the Q/R site CAG codon and, additionally, in intronic adenosines. Transfection of GluR-B gene constructs into PC12 cells revealed that the proximal part of the intron downstream of the unedited exonic site is required for Q/R site editing. This intron portion contains an imperfect inverted repeat preceding a 10 nt sequence with exact complementarity to the exon centered on the unedited codon. Single nucleotide substitutions in this short intronic sequence or its exonic complement curtailed Q/R site editing, which was recovered by restoring complementarity in the respective partner strand. Base conversion in the channel-coding region of GluR-B directed by base paired sequences may be executed by a ubiquitous nuclear adenosine deaminase specific for double-stranded RNA.

MeSH terms

  • Adenosine Deaminase / metabolism
  • Animals
  • Base Sequence
  • Gene Expression
  • Genes
  • Introns
  • Mice
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Oligodeoxyribonucleotides / chemistry
  • PC12 Cells
  • RNA Editing*
  • RNA Processing, Post-Transcriptional
  • RNA Splicing
  • RNA, Messenger / genetics
  • Receptors, AMPA / genetics*
  • Repetitive Sequences, Nucleic Acid
  • Restriction Mapping


  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Receptors, AMPA
  • Adenosine Deaminase