Induction of axonal growth by heterophilic interactions between the cell surface recognition proteins F11 and Nr-CAM/Bravo

Neuron. 1993 Dec;11(6):1113-22. doi: 10.1016/0896-6273(93)90224-f.

Abstract

F11 and Nr-CAM/Bravo are two axon-associated glycoproteins belonging to different subgroups of the immunoglobulin superfamily. In this report we have investigated the interaction of both proteins using neurite outgrowth and binding assays. Antibody blocking experiments demonstrate that neurite extension of tectal cells on immobilized F11 is mediated by Nr-CAM/Bravo. Binding studies further reveal a direct heterophilic interaction between F11 and Nr-CAM/Bravo. This activity can be mapped to the amino-terminal second or third immunoglobulin-like domain within F11 with domain-specific monoclonal antibodies and deletion mutant proteins expressed on COS cells. Furthermore, perturbation experiments with domain-specific monoclonal antibodies demonstrate that this region is required for adhesion and neurite extension.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology
  • Axons / physiology*
  • Cell Adhesion
  • Cell Adhesion Molecules*
  • Cell Adhesion Molecules, Neuronal / immunology
  • Cell Adhesion Molecules, Neuronal / metabolism*
  • Cell Line
  • Cell Membrane / metabolism
  • Cell Membrane / physiology
  • Contactins
  • Flow Cytometry
  • Membrane Glycoproteins / metabolism*
  • Mice
  • Mice, Inbred BALB C / immunology
  • Microspheres
  • Nerve Growth Factors / metabolism
  • Nerve Tissue Proteins / metabolism*
  • Neural Cell Adhesion Molecules*
  • Neurites / physiology*
  • Protein Binding
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / metabolism
  • Superior Colliculi / metabolism
  • Superior Colliculi / physiology*
  • Transfection

Substances

  • Antibodies, Monoclonal
  • Bravo protein, Gallus gallus
  • Cell Adhesion Molecules
  • Cell Adhesion Molecules, Neuronal
  • Contactins
  • Membrane Glycoproteins
  • Nerve Growth Factors
  • Nerve Tissue Proteins
  • Neural Cell Adhesion Molecules
  • Nfasc protein, mouse
  • Nrcam protein, mouse
  • Recombinant Proteins