Transcription in vitro of phi29 DNA and EcoRI fragments by Bacillus subtilis RNA polymerase

Eur J Biochem. 1976 Dec;71(1):77-83. doi: 10.1111/j.1432-1033.1976.tb11091.x.


EcoRI fragments A, B and C produced from linear phi29 DNA, but not D or E fragments, are transcribed by purified Bacillus subtilis RNA polymerase. The transcription of fragments A and C is initiated preferentially with GTP and to a lesser extent with ATP; the reverse happens in the case of fragment B. The dinucleotides GpU and GpA respectively, compete specifically with the incorporation of [gamma-32P]GTP directed by fragments A and C. The RNA synthesized in vitro by purified B. subtilis RNA polymerase is highly asymmetric. Most of the RNA synthesis directed by fragments A and C is early RNA. However, most of the RNA produced by fragment B is anti-late-RNA. Addition of crude extracts inhibit the transcription of fragment B but not that of fragments A and C.

MeSH terms

  • Bacillus subtilis / enzymology*
  • Bacteriophages / metabolism*
  • DNA, Bacterial / metabolism*
  • DNA, Viral / metabolism*
  • DNA-Directed RNA Polymerases / metabolism*
  • Escherichia coli
  • Kinetics
  • Transcription, Genetic*


  • DNA, Bacterial
  • DNA, Viral
  • DNA-Directed RNA Polymerases