Comparison of monoclonal antibodies AUA1 and BER EP4 with anti-CEA for detecting carcinoma cells in serous effusions and distinguishing them from mesothelial cells

Cytopathology. 1993;4(5):267-71. doi: 10.1111/j.1365-2303.1993.tb00101.x.


Commercially available monoclonal antibodies AUA1, BER EP4 and carcinoembryonic antigen (CEA) were applied to cell blocks from 95 serous effusions. AUA1 and BER EP4 were reactive with 89% of effusions known to contain carcinoma cells, and anti-CEA with 71%. They also reacted with cells in two effusions from patients with malignant disease which were regarded as negative on conventional cytological examination of Papanicolaou-stained smears. They were negative in all but one of the benign effusions. Using all three antibodies, 95% of effusions containing carcinoma cells were detected. Use of these antibodies could improve the cytological diagnosis of serous effusions.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Monoclonal*
  • Ascitic Fluid / chemistry
  • Ascitic Fluid / pathology*
  • Carcinoembryonic Antigen / analysis*
  • Carcinoma / chemistry
  • Carcinoma / pathology*
  • Epithelial Cells
  • Humans


  • Antibodies, Monoclonal
  • Carcinoembryonic Antigen