Isolation and characterization of a gene for a ryanodine receptor/calcium release channel in Drosophila melanogaster

FEBS Lett. 1994 Jan 3;337(1):81-7. doi: 10.1016/0014-5793(94)80634-9.


The nucleotide sequence of a 25.7 kilobase Drosophila melanogaster genomic DNA segment containing a gene for a ryanodine receptor/calcium release channel homologue has been determined. Computer analysis and partial cDNA cloning revealed 26 exons comprising the protein-coding sequence in this gene. The predicted protein is homologous in amino acid sequence and shares characteristic structural features with the mammalian ryanodine receptors. In blot hybridization analysis, a approximately 16 kilobase RNA species was identified abundantly in a 6-12 h embryo as the transcript from this gene. In situ hybridization to polytene chromosomes indicated that this gene locates at band position 44F on the second chromosome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Calcium Channels / chemistry
  • Calcium Channels / genetics*
  • Chromosome Mapping
  • Chromosomes / chemistry
  • Cloning, Molecular
  • DNA / chemistry
  • DNA / isolation & purification*
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • Drosophila melanogaster / genetics*
  • Endoplasmic Reticulum / chemistry
  • In Situ Hybridization
  • Molecular Sequence Data
  • Muscle Proteins / chemistry
  • Muscle Proteins / genetics*
  • Nucleic Acid Hybridization
  • Repetitive Sequences, Nucleic Acid
  • Restriction Mapping
  • Ryanodine Receptor Calcium Release Channel


  • Calcium Channels
  • DNA, Complementary
  • Muscle Proteins
  • Ryanodine Receptor Calcium Release Channel
  • DNA

Associated data

  • GENBANK/D17389