The human cytomegalovirus 86K immediate early (IE) 2 protein requires the basic region of the TATA-box binding protein (TBP) for binding, and interacts with TBP and transcription factor TFIIB via regions of IE2 required for transcriptional regulation

J Gen Virol. 1993 Dec;74 ( Pt 12):2691-8. doi: 10.1099/0022-1317-74-12-2691.

Abstract

The 86K immediate early (IE) 2 protein of human cytomegalovirus trans-activates a number of homologous and heterologous promoters, including the cellular promoter for the 70K heat-shock protein (hsp70), and the human immunodeficiency virus long terminal repeat. We have previously shown that IE2 trans-activates these two promoters in a TATA-dependent manner, and that IE2 is able to form a direct contact with TATA-box binding protein (TBP) in vitro. We now show that IE2 binds to the basic repeat region of TBP. In addition IE2 can contact a second general transcription factor, TFIIB. We have mapped the TBP- and TFIIB-binding regions within IE2 and show that these regions overlap, and also lie within parts of the protein previously identified as being required for the trans-activation and autoregulation functions of IE2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cytomegalovirus / genetics*
  • DNA Mutational Analysis
  • DNA-Binding Proteins / metabolism*
  • Humans
  • Immediate-Early Proteins / metabolism*
  • Membrane Glycoproteins*
  • Molecular Sequence Data
  • Recombinant Fusion Proteins / metabolism
  • Repetitive Sequences, Nucleic Acid
  • TATA-Box Binding Protein
  • Trans-Activators*
  • Transcription Factor TFIIB
  • Transcription Factors / metabolism*
  • Transcriptional Activation*
  • Viral Envelope Proteins*
  • Viral Proteins*

Substances

  • DNA-Binding Proteins
  • IE2 protein, Cytomegalovirus
  • Immediate-Early Proteins
  • Membrane Glycoproteins
  • Recombinant Fusion Proteins
  • TATA-Box Binding Protein
  • Trans-Activators
  • Transcription Factor TFIIB
  • Transcription Factors
  • UL115 protein, Human herpesvirus 5
  • Viral Envelope Proteins
  • Viral Proteins
  • glycoprotein H, Cytomegalovirus
  • glycoprotein H, Human cytomegalovirus
  • glycoprotein O, cytomegalovirus