Stimulation of murine lymphocyte proliferation by a phosphorothioate oligonucleotide with antisense activity for herpes simplex virus

Life Sci. 1994;54(2):101-7. doi: 10.1016/0024-3205(94)00780-2.


To investigate further the immunological properties of nucleic acids, the mitogenicity of a phosphorothioate oligonucleotide (S-oligo 1082) with antisense activity for herpes simplex virus was tested. This compound stimulated proliferation and antibody production by murine lymphocytes in in vitro cultures. Proliferation was dose-dependent and unaffected by T cell depletion. Furthermore, inclusion of a non-mitogenic DNA in the medium did not block stimulation. Since 1082 does not have homology to a known gene involved in lymphocyte activation, these observations suggest that S-oligo antisense compounds may display non-specific activating effects, at least on murine B cells.

MeSH terms

  • Animals
  • Antibody Formation / drug effects
  • B-Lymphocytes / drug effects*
  • B-Lymphocytes / physiology
  • Base Sequence
  • Cells, Cultured
  • Lymphocyte Activation / drug effects
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides, Antisense*
  • Oligonucleotides, Antisense / pharmacology*
  • Simplexvirus / drug effects*
  • Thionucleotides / pharmacology*


  • Oligodeoxyribonucleotides, Antisense
  • Oligonucleotides, Antisense
  • Thionucleotides
  • ISIS 1082