Construction of a transcription map of a 300 kb region around the human G6PD locus by direct cDNA selection

Hum Mol Genet. 1993 Nov;2(11):1865-9. doi: 10.1093/hmg/2.11.1865.


A transcription map covering a 300 kb region around the G6PD gene in the human Xq28 region was constructed by the direct cDNA selection method and the analysis of the resulting region-specific enriched cDNA sublibrary. Seven new genes and two loci of endogenous retrovirus HERV-K were identified. The distribution of the genes across the region is strongly non-uniform and follows the non-uniform distribution of GpG islands in the area. While one of the novel genes was found to be highly homologous to bovine smg p25A GDP-dissociation inhibitor, the remaining genes did not detect any homology to known genes. The analysis of region-specific cDNA sublibraries represents a simple, rapid and efficient tool for the generation of a regional transcription map.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Chromosome Mapping
  • DNA, Complementary / analysis*
  • Dinucleoside Phosphates / analysis
  • GTP-Binding Proteins / genetics
  • Gene Library
  • Glucosephosphate Dehydrogenase / biosynthesis
  • Glucosephosphate Dehydrogenase / genetics*
  • Humans
  • Molecular Sequence Data
  • Organ Specificity
  • Restriction Mapping
  • Retroviridae / genetics
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic*
  • X Chromosome*
  • rab3 GTP-Binding Proteins


  • DNA, Complementary
  • Dinucleoside Phosphates
  • cytidylyl-3'-5'-guanosine
  • Glucosephosphate Dehydrogenase
  • GTP-Binding Proteins
  • rab3 GTP-Binding Proteins

Associated data

  • GENBANK/X74605
  • GENBANK/X74606
  • GENBANK/X74607
  • GENBANK/X74608
  • GENBANK/X74609
  • GENBANK/X74610
  • GENBANK/X74611
  • GENBANK/X74612
  • GENBANK/X74613