The anther-specific protein encoded by the Brassica napus and Arabidopsis thaliana A6 gene displays similarity to beta-1,3-glucanases

Plant J. 1993 Dec;4(6):1023-33. doi: 10.1046/j.1365-313x.1993.04061023.x.

Abstract

An anther-specific Brassica napus cDNA, A6, and two corresponding Arabidopsis thaliana genes have been isolated. Sequence analyses of A6 revealed similarity to beta-1,3-glucanases. The deduced A6 protein differs from other beta-1,3-glucanases in the possession of a long C-terminus. Immunoblotting using an antibody raised to the A6 protein detects a temporal 60 kDa protein in B. napus buds, suggesting that the long C-terminal region is present in the mature protein. A6 promoter-GUS and RNase fusions demonstrate that the A6 gene is tapetum-specific and temporally expressed with a peak in activity when the plant normally expresses callase (a complex of endo- and exo-beta-1,3-glucanase activities). The sequence similarity of A6 to other beta-1,3-glucanases, coupled with the temporal and spatial expression data, suggests that A6 may be part of the callase enzyme complex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / enzymology
  • Arabidopsis / genetics*
  • Arabidopsis Proteins*
  • Base Sequence
  • Brassica / enzymology
  • Brassica / genetics*
  • Cloning, Molecular
  • DNA
  • Fluorometry
  • Gene Expression
  • Genes, Plant
  • Glucan 1,3-beta-Glucosidase
  • Glucan Endo-1,3-beta-D-Glucosidase / genetics
  • Glucan Endo-1,3-beta-D-Glucosidase / metabolism
  • Immunoblotting
  • Molecular Sequence Data
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Plants, Toxic
  • Sequence Homology, Amino Acid
  • Tobacco / genetics
  • Tobacco / metabolism
  • beta-Glucosidase / genetics*
  • beta-Glucosidase / metabolism

Substances

  • A6 protein, Arabidopsis
  • Arabidopsis Proteins
  • Plant Proteins
  • DNA
  • beta-Glucosidase
  • Glucan Endo-1,3-beta-D-Glucosidase
  • Glucan 1,3-beta-Glucosidase

Associated data

  • GENBANK/X69887
  • GENBANK/X69888
  • GENBANK/X69889
  • GENBANK/X69890
  • GENBANK/X70409