[3H]7-OH-DPAT labels both dopamine D3 receptors and sigma sites in the bovine caudate nucleus

Eur J Pharmacol. 1993 Oct 5;242(3):R1-2. doi: 10.1016/0014-2999(93)90259-k.


The binding of [3H]7-hydroxy-N,N-di-n-propyl-2-aminotetralin (7-OH-DPAT) to membranes of the bovine caudate nucleus was characterized and shown to be heterogenous. While [3H]7-OH-DPAT labels the dopamine D3 receptor in this tissue (Ki = 4.8 nM), as shown by its sensitivity to dopamine and (-)-quinpirole, it also binds with high affinity (Ki = 48 nM) to sigma recognition sites. The present results demonstrate that [3H]7-OH-DPAT is a useful radioligand to label native D3 receptors in the bovine caudate, although care must be taken to assure its selectivity.

MeSH terms

  • Animals
  • Cattle
  • Caudate Nucleus / metabolism*
  • Radioligand Assay
  • Receptors, Dopamine / metabolism*
  • Receptors, Dopamine D2*
  • Receptors, Dopamine D3
  • Receptors, sigma / metabolism*
  • Tetrahydronaphthalenes / metabolism*
  • Tritium


  • Receptors, Dopamine
  • Receptors, Dopamine D2
  • Receptors, Dopamine D3
  • Receptors, sigma
  • Tetrahydronaphthalenes
  • Tritium
  • 7-hydroxy-2-N,N-dipropylaminotetralin