A new imprinted gene cloned by a methylation-sensitive genome scanning method

Nucleic Acids Res. 1993 Dec 11;21(24):5577-82. doi: 10.1093/nar/21.24.5577.

Abstract

We cloned a new imprinted gene by searching for parental-origin-specific CpG methylations using methylation-sensitive two-dimensional genome scanning method. This gene encodes a putative 51 kDa protein with significant similarity to U2 small nuclear ribonucleoprotein auxiliary factor small subunits, an essential mammalian splicing factor, and is located on mouse chromosome 11, of which maternal duplication/paternal deficiency results in a small body.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Blotting, Southern
  • Cloning, Molecular / methods*
  • DNA / genetics
  • DNA / metabolism*
  • Dinucleoside Phosphates / metabolism*
  • Genome
  • Methylation
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics*
  • Nuclear Proteins*
  • Polymerase Chain Reaction
  • Restriction Mapping
  • Ribonucleoproteins*
  • Ribonucleoproteins, Small Nuclear / genetics*
  • Splicing Factor U2AF

Substances

  • Dinucleoside Phosphates
  • Nerve Tissue Proteins
  • Nuclear Proteins
  • Ribonucleoproteins
  • Ribonucleoproteins, Small Nuclear
  • Splicing Factor U2AF
  • Zrsr1 protein, mouse
  • cytidylyl-3'-5'-guanosine
  • DNA

Associated data

  • GENBANK/D17407