Serum antibody responses against shigella lipopolysaccharides and invasion plasmid-coded antigens in shigella infected Swedish patients

Scand J Infect Dis. 1993;25(5):569-77. doi: 10.3109/00365549309008545.


Serum antibody responses to shigella lipopolysaccharide (LPS) and invasion plasmid-coded antigens (Ipa) were studied in 74 Swedish patients with culture verified bacillary dysentery using class-specific enzyme immunoassay (EIA). Anti-LPS responses were found in 80% and 79% serum samples, respectively, from S. flexneri and S. sonnei infected patients and anti-Ipa responses in 60% and 43%, respectively. The mean anti-Ipa IgG antibody titres in S. flexneri infected patients remained high for 4-6 months after onset while the anti-LPS IgG antibody titres had dropped to normal levels. The specificity of EIA for shigella Ipa was 90% and for S. dysenteriae, S. flexneri and S. sonnei LPSs it varied between 84% and 90%. No close correlations between the anti-LPS and anti-Ipa antibody responses were observed indicating that they may be differently regulated. The dynamics of the serum antibody responses indicates that an anti-LPS response is a good indicator of a recent shigella infection and an anti-Ipa IgG response a good indicator of a previous infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies, Bacterial / blood*
  • Antigens, Bacterial / immunology*
  • Bacterial Proteins*
  • Dysentery, Bacillary / blood
  • Dysentery, Bacillary / immunology*
  • Humans
  • Immunoenzyme Techniques
  • Immunoglobulin A / blood
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Lipopolysaccharides / immunology*
  • Sensitivity and Specificity
  • Shigella dysenteriae / immunology*
  • Shigella flexneri / immunology*
  • Shigella sonnei / immunology*
  • Sweden


  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Proteins
  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M
  • IpaA protein, Shigella flexneri
  • Lipopolysaccharides