Search for Mycobacterium paratuberculosis DNA in tissue from patients with sarcoidosis by enzymatic gene amplification

APMIS. 1993 Nov;101(11):876-8. doi: 10.1111/j.1699-0463.1993.tb00195.x.


The etiology of sarcoidosis has not yet been established, but several mycobacterial species have been implicated in the pathogenesis of this disease. We have used a nested primer polymerase chain reaction (PCR) specific for the IS900 Mycobacterium paratuberculosis insertion element to analyse paraffin-embedded mediastinal lymphoid tissue from 18 patients with pulmonary sarcoidosis verified by mediastinoscopy. Only the positive control contained M. paratuberculosis-specific DNA. The present study does not support a role for M. paratuberculosis in the pathogenesis of sarcoidosis. However, further studies are needed in order to clarify the possible role of other mycobacteria in this disease.

MeSH terms

  • Adolescent
  • Adult
  • Base Sequence
  • DNA Primers / chemistry
  • DNA, Bacterial / analysis
  • Female
  • Humans
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Mycobacterium avium subsp. paratuberculosis*
  • Polymerase Chain Reaction
  • Sarcoidosis / microbiology*


  • DNA Primers
  • DNA, Bacterial