HNF-3 beta as a regulator of floor plate development

Cell. 1994 Jan 14;76(1):103-15. doi: 10.1016/0092-8674(94)90176-7.

Abstract

The transcription factor gene HNF-3 beta is expressed in the ventral midline of the mouse embryonic neural tube, including the floor plate, a structure important for dorsoventral patterning and axonal guidance. To assess HNF-3 beta function, the gene has been ectopically expressed in the midbrain/hindbrain of transgenic embryos using an En-2 promoter/enhancer. By 18.5 days postcoitum, transgenic brains show a range of abnormalities, including absent inferior colliculus and reduced cerebellum. Earlier, several genes normally expressed in the floor plate (BMP-1, Steel factor, and HNF-3 alpha) are induced within the same ectopic dorsal domain as HNF-3 beta, and autoactivation of the endogenous HNF-3 beta is observed. Conversely, expression of the dorsal gene Pax-3 is suppressed. Ectopic dorsal neuronal differentiation and abnormal dorsal axonal projections are also seen. These results suggest that HNF-3 beta is an important regulator of floor plate development in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Axons / physiology
  • Base Sequence
  • Cloning, Molecular
  • DNA-Binding Proteins / biosynthesis*
  • Embryo, Mammalian / physiology*
  • Embryonic and Fetal Development
  • Exons
  • Gene Expression Regulation
  • Gene Expression*
  • Globins / biosynthesis
  • Globins / genetics
  • Hepatocyte Nuclear Factor 3-beta
  • In Situ Hybridization
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • Nervous System / embryology*
  • Nuclear Proteins / biosynthesis*
  • Oligodeoxyribonucleotides
  • Oligonucleotide Probes
  • Phenotype
  • Polymerase Chain Reaction
  • Rabbits
  • Transcription Factors / biosynthesis*

Substances

  • DNA-Binding Proteins
  • Foxa2 protein, mouse
  • Nuclear Proteins
  • Oligodeoxyribonucleotides
  • Oligonucleotide Probes
  • Transcription Factors
  • Hepatocyte Nuclear Factor 3-beta
  • Globins

Associated data

  • GENBANK/L25669
  • GENBANK/L25670