Assignment of the human PAX4 gene to chromosome band 7q32 by fluorescence in situ hybridization

Cytogenet Cell Genet. 1994;66(2):132-4. doi: 10.1159/000133684.


Of the nine known members of a human paired box-containing gene family (Pax), only PAX4 has not been precisely localized. We screened a cosmid library of human genomic DNA using polymerase chain reaction products for PAX4 as a probe and isolated three positive cosmid clones. Sequence analysis revealed that at least two of them had exon-like sequences and showed extensive homology to Pax-4 in the mouse. These two cosmid clones were mapped to human chromosome band 7q32 by fluorescence in situ hybridization.

MeSH terms

  • Animals
  • Base Sequence
  • Chromosome Banding
  • Chromosomes, Human, Pair 7*
  • Cloning, Molecular
  • Cosmids
  • DNA
  • DNA-Binding Proteins / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Mice
  • Molecular Sequence Data
  • Polymerase Chain Reaction


  • DNA-Binding Proteins
  • DNA