Abstract
This paper presents a new method for site-specific labelling of antibodies employing enzymatic reactions without oxidizing or reducing agents. IgG was first treated with immobilized sialidase from Clostridium perfringens to cleave bound NeuAc. CMP-9-deoxy-9-salizoyl-NeuAc, an activated sialic acid analogue, was labelled with 131I via the iodogen-method in high yields (> 95%). Then the oligosaccharide chains of antibodies were labelled yield with the radioactive NeuAc analogue by transfer using alpha-2,6-sialyltransferase from rat liver in 50%.
MeSH terms
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Animals
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Antibodies*
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Carbon Radioisotopes
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Cytidine Monophosphate N-Acetylneuraminic Acid
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Immunoglobulin G*
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Indicators and Reagents
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Iodine Radioisotopes
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Isotope Labeling / methods*
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Kinetics
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Liver / enzymology
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Neuraminidase / metabolism
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Oligosaccharides*
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Rats
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Sialic Acids*
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Sialyltransferases / metabolism
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beta-D-Galactoside alpha 2-6-Sialyltransferase
Substances
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Antibodies
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Carbon Radioisotopes
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Immunoglobulin G
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Indicators and Reagents
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Iodine Radioisotopes
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Oligosaccharides
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Sialic Acids
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Cytidine Monophosphate N-Acetylneuraminic Acid
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Sialyltransferases
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Neuraminidase
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beta-D-Galactoside alpha 2-6-Sialyltransferase