Islet-specific T-cell clones from the NOD mouse respond to beta-granule antigen

Diabetes. 1994 Feb;43(2):197-203. doi: 10.2337/diab.43.2.197.


Islet-reactive T-cell clones from NOD mice provide an important approach to the investigation of antigens with relevance to type I diabetes. To identify a source of beta-cell antigen suitable for biochemical studies, we have used two islet-specific, diabetogenic T-cell clones to test beta-tumor cells. beta-tumor cell lines, maintained in continuous culture, were found to lose antigenicity rapidly. However, cells harvested directly from beta-tumors arising spontaneously in the transgenic NOD/Lt-Tg(RIPTag)1Lt mouse proved to be a potent source of beta-cell antigen for the T-cell clones. Subcellular fractionation of beta-tumor cells showed that the T-cell antigen was highly enriched in the beta-granule fraction and that this activity was associated with the granule membrane.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Neoplasm / immunology*
  • Cell Fractionation / methods
  • Cell Line
  • Centrifugation, Density Gradient
  • Clone Cells
  • Culture Techniques / methods
  • Cytoplasmic Granules / immunology*
  • Diabetes Mellitus, Type 1 / genetics
  • Diabetes Mellitus, Type 1 / immunology*
  • Humans
  • Insulinoma / immunology*
  • Islets of Langerhans / immunology*
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred NOD / immunology
  • Mice, Inbred Strains / immunology
  • Pancreatic Neoplasms / immunology*
  • Rats
  • T-Lymphocytes / immunology*
  • Tumor Cells, Cultured


  • Antigens, Neoplasm