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, 114 (3), 349-54

Complementation of Growth Defect in an ampC Deletion Mutant of Escherichia Coli

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Complementation of Growth Defect in an ampC Deletion Mutant of Escherichia Coli

R E Bishop et al. FEMS Microbiol Lett.

Abstract

beta-Lactamase genes of class-A (Rtem) and class-C (ampC) were placed under control of an inducible tac-promoter and expressed in Escherichia coli. Expression of RTEM had no observable effect on the growth properties of E. coli strains HB101 (ampC+) or MI1443 (delta ampC). E. coli MI1443 exhibited a decline in growth rate at mid-exponential phase which could be delayed by expression of AmpC at early-exponential phase. AmpC expression otherwise inhibited growth, particularly during the transition into exponential phase where growth was prevented altogether. We suggest that the AmpC beta-lactamase, but not RTEM, may have an additional cellular function as a peptidoglycan hydrolase.

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