Localization of the gene encoding peptidylglycine alpha-amidating monooxygenase (PAM) to human chromosome 5q14-5q21

Genomics. 1993 Nov;18(2):319-21. doi: 10.1006/geno.1993.1471.

Abstract

Peptidylglycine alpha-amidating monooxygenase (PAM; EC 1.14.17.3) is a multifunctional protein containing two enzymes that act sequentially to catalyze the alpha-amidation of neuroendocrine peptides. Southern blot analysis of human placental DNA demonstrated that PAM is encoded by a single gene. The chromosomal localization of the PAM gene was established using in situ hybridization. A 2.2-kb human PAM cDNA hybridized to human metaphase chromosomes revealed a significant clustering of silver grains over chromosome 5 bands q14-q21. The gene encoding another enzyme important in the post-translational processing of neuroendocrine precursors, prohormone convertase 1 (PC1), is localized in the same region (5q15-q21).

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Blotting, Southern
  • Cells, Cultured
  • Chromosome Banding
  • Chromosome Mapping
  • Chromosomes, Human, Pair 5*
  • Humans
  • Mixed Function Oxygenases / genetics*
  • Multienzyme Complexes*

Substances

  • Multienzyme Complexes
  • Mixed Function Oxygenases
  • peptidylglycine monooxygenase