Loss in expression of the retinoblastoma gene product in human gliomas is associated with advanced disease

J Neurooncol. 1993 May;16(2):159-65. doi: 10.1007/BF01324703.

Abstract

The retinoblastoma susceptibility gene (RB), the prototype of the class of tumor suppressor genes, is inactivated in a number of human malignancies. We investigated a possible role of RB in human brain tumors. Immunoprecipitation revealed frequent loss of RB protein expression in glioma cell lines (8/24), which was accompanied by lack of RB encoded transcripts. Among seventeen primary brain tumors studied by Western blotting, loss of Rb protein expression was observed in WHO grade 3 and 4 gliomas (3/10). However, none of the low grade gliomas and the other primary brain tumors investigated lacked RB protein expression. These data suggest a role for RB in glial malignancy, and loss of Rb expression appears to be associated with glial tumor progression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Brain Neoplasms / metabolism*
  • Child, Preschool
  • Female
  • Glioma / metabolism*
  • Humans
  • Male
  • Middle Aged
  • Prognosis
  • Retinoblastoma Protein / biosynthesis*
  • Tumor Cells, Cultured

Substances

  • Retinoblastoma Protein