Increased longevity of EF-1 alpha high-fidelity mutants in Podospora anserina

J Mol Biol. 1994 Jan 7;235(1):231-6. doi: 10.1016/s0022-2836(05)80029-4.


Various translation initiation and elongation factors seem to participate in the control of the cellular proliferation and the ageing process in higher eukaryotes. Studies indicate that EF-1 alpha, one of the translation elongation factors, may be one of the major components involved. We here present the cloning of the filamentous fungus P. anserina EF-1 alpha encoding gene and show that strains bearing high fidelity mutations in the EF-1 alpha gene have a drastically increased longevity as well as an impairment in sporulation. This suggests that EF-1 alpha involved in the sexual and senescence processes in lower eukaryotes, through the control of translational errors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA Primers
  • DNA, Fungal / analysis
  • Genes, Fungal*
  • Molecular Sequence Data
  • Mutation
  • Peptide Elongation Factor 1
  • Peptide Elongation Factors / genetics
  • Peptide Elongation Factors / metabolism*
  • Polymerase Chain Reaction
  • Protein Biosynthesis
  • Xylariales / genetics
  • Xylariales / metabolism
  • Xylariales / physiology*


  • DNA Primers
  • DNA, Fungal
  • Peptide Elongation Factor 1
  • Peptide Elongation Factors

Associated data

  • GENBANK/X74799