Differentially spliced erg-3 product functions as a transcriptional activator

Oncogene. 1994 Feb;9(2):669-73.


ets oncogene superfamily consists of a family of transcriptional factors that functions as activators and/or repressors. Previously, we have identified a member of this ets superfamily namely erg, ets related gene. erg gene was shown to code for at least two proteins erg-1 and erg-2 because of alternative splicing and alternative usage of initiation codon. In this report we show that erg gene codes for an additional erg variant protein, erg-3 as a result of differential splicing which results in the insertion of 24 amino acids in the coding region of erg-2 protein. RNAase protection analysis revealed that erg-3 transcripts are expressed in a variety of cells. Erg-3 was also found to activate the transcription of the reporter TK-CAT gene linked to erg target sequences suggesting that erg-3 codes for a sequence specific transcriptional activator.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA / genetics
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / physiology*
  • Genes, Regulator / genetics
  • Genes, Regulator / physiology
  • Genes, Reporter / genetics
  • Humans
  • Molecular Sequence Data
  • Trans-Activators*
  • Transcription Factors / genetics
  • Transcription Factors / physiology*
  • Transcription, Genetic / genetics
  • Transcription, Genetic / physiology*
  • Transcriptional Activation / genetics
  • Transcriptional Activation / physiology*
  • Transcriptional Regulator ERG


  • DNA-Binding Proteins
  • ERG protein, human
  • Trans-Activators
  • Transcription Factors
  • Transcriptional Regulator ERG
  • DNA

Associated data

  • GENBANK/S68130